Use of dual TaqMan probes to increase the sensitivity of 1-step quantitative reverse transcription-PCR: application to the detection of SARS coronavirus.

Authors:
Yip SP; To SS; Leung PH; Cheung TS; Cheng PK and 1 more

Journal:
Clin Chem

Publication Year: 2005

DOI:
10.1373/clinchem.2005.054106

PMCID:
PMC7108148

PMID:
16189379

Journal Information

Full Title: Clin Chem

Abbreviation: Clin Chem

Country: Unknown

Publisher: Unknown

Language: N/A

Publication Details

Subject Category: Chemistry, Clinical

Available in Europe PMC: Yes

Available in PMC: Yes

PDF Available: No

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Evidence found in paper:

"This work was supported by a Hong Kong Research Grant Council Special Grant for SARS Research (PolyU 5520/03M). Purchase of the 7500 Real Time PCR System was supported by a Big Equipment Grant (G.53.27.D032) to S.P.Y., S.S.T.T., and P.H.M.L. by the Hong Kong Polytechnic University. We thank Lee Sau Yin for help in aligning the SARS-CoV genome sequences and Karen Anne Rocha for setting up the P1 assay in the initial stage."

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Last Updated: Aug 05, 2025