Identification of a porcine DC-SIGN-related C-type lectin, porcine CLEC4G (LSECtin), and its order of intron removal during splicing: comparative genomic analyses of the cluster of genes CD23/CLEC4G/DC-SIGN among mammalian species.

"the primer sequences were based on the s scrofa chromosome 2 clone ch242-334a8 (working draft sequence; genbank accession number cu929919 ) that was released most recently on september 24 2008.; species gene cd23 (fcer2) lsectin (clec4g) dc-sign (cd209) l-sign (cd209l) unigene # or symbol genbank accession # unigene # or symbol genbank accession # unigene # or symbol genbank accession # unigene # or symbol genbank accession # human ( homo sapiens ) hs465778 nc_000019 (gene) nm_002002 (mrna) hs220649 hs568222 (pseudogene) nc_000019 (gene) nm_198492 (mrna) nr_002931 (non-coding rna from pseudogene) hs278694 nc_000019 (gene) nm_021155 (mrna) hs421437 nc_000019 (gene) nm_014257 (isoform1 mrna) chimpanzee ( pan troglodytes ) loc456434 nc_006486 (pseudogene) xr_023699 (non-coding rna from pseudogene) clec4g nc_006486 (gene) xm_001154312 (mrna) xr_023719 (non-coding rna from pseudogene) cd209 nc_006486 (gene) nm_001009064 (mrna) cd209l1 cd209l2 nc_006486 (genes) xm_512333 ( cd209l1 mrna) xm_001146279 ( cd209l2 mrna) rhesus macaque ( macaca mulatta ) fcer2 nc_007876 (gene) xm_001097471 (incomplete mrna) loc704872 (pseudogene1) loc705334 (pseudogene2) nc_007876 (gene) xr_011056 and xr_011166 (non-coding rnas from pseudogenes1 and 2) mmu 3596 nc_007876 (gene) nm_001032870 (isoform1 mrna) mmu 3664 nc_007876 (gene) nm_001032951 ( cd209l2 mrna) cattle ( bos taurus ) bt 29723 nc_007305 (gene) xm_592551 (mrna) bt 22180 nc_007305 (gene) xm_583924 (mrna) bt 9532 nc_007305 (gene) xm_590928 (mrna) none none dog ( canis lupus ) cfa 41676 nc_006602 (gene) xm_542116 (mrna) cfa 41675 nc_006602 (gene) xm_542117 (mrna) cfa 14490 nc_006602 (gene) xm_542118 (mrna) none none horse ( equus caballus ) eca 13012 nc_009150 (gene) nm_001081807 (mrna) loc100066617 (clec4g1) eca 14020 (clec4g2) loc100066686 (clec4g3) nc_009150 (genes) xm_001496835 xm_001496849 xm_001496883 (clec4gs1-3 mrnas) loc100066738 nc_009150 (genes) xm_001496929 (mrna) none none pig ( sus scrofa ) unassigned cu929919 (gene) fj545265 (mrna) ssc 19137 cu929919 or eu814899 (gene) ak232603 (mrna) ssc 9743 cu929919 (gene) nm_001129972 or eu684956 (mrna) none none sheep ( ovis aries ) oar 8217 ee816942 (incomplete mrna) oar 10271 ee825489 and ee789989 overlapping (complete mrna) unassigned ee825175 (mrna) none none opossum ( monodelphis domestica ) loc100026894 nc_008803 (gene) xm_001377323 (mrna) loc100026825 (clec4g1) loc100013537 (clec4g2) nc_008803 (genes) xm_001377275 and xm_001367882 (clec4gs1 and 2 mrnas) loc100026844 (dc-sign1) loc100026862 (dc-sign2) nc_008803 (genes) xm_001377290 and xm_001377303 (dc-signs1 and 2 mrnas) none none duck-billed platypus ( ornithorhynchus anatinus ) unknown unknown loc100081235 (clec4g1) loc100081208 (clec4g2) nw_001765451 (gene) xm_001512010 and xm_001511987 (clec4gs1 and 2 mrnas) unknown unknown unknown unknown mouse ( mus musculus ) mm 1233 nc_000074 (gene) nm_013517 (mrna) mm 109183 nc_000074 (gene) nm_029465 (mrna) signr (cd209) unigene # or symbol genbank accession # mm 175163 (signr1) mm 215729 (signr2) mm 111026 (signr3) mm 52281 (signr4) mm 32510 (signr5) mm 390006 (signr7) mm 171061 (signr8) nc_000074 (genes) mrnas: nm_026972 (signr1) nm_130903 (signr2) nm_130904 (signr3) nm_130905 (signr4) nm_133238 (signr5) xm_284376 (signr7) xm_284386 (signr8) rat ( rattus norvegicus ) rn 10326 nc_005111 (gene) nm_001033924 (mrna) clec4g nc_005111 (gene) xm_001069149 (mrna) rn 138846 (signr1) cd209c (signr2) rn 218629 (signr3) rn 220862 (signr4) rn 218460 (signr5) rn 198282 (signr2-r1) loc688858 (signr2-r2) rn 214149 (signr2-r3) rgd1561234 (signr2-r4) rgd1564563 (signr2-r5) rgd1564571 (signr2-r6) rn 137828 (signr7) nc_005111 (genes) mrnas: xm_213687 (signr1) xm_001068950 (signr2) xm_344064 (sign3) xm_577206 (sign4) xm_221778 (signr5) xm_001068850 (signr2-r1) xm_001068599 (signr2-r2) xm_221789 (signr2-r3) xm_221790 (signr2-r4) xm_577205 (signr2-r5) xm_221808 (signr2-r6) xm_001068161 (signr7) phylogenetic tree was constructed by the neighbor-joining method in the paup 4 0 program (david swofford smithsonian institute washington dc distributed by sinauer associate inc) based upon the available complete amino acid coding sequences of cd23 clec4g and dc-sign family proteins.; extra nucleotide sequences at both termini in the non-coding region of pclec4g cdna that were not determined in this study were included with dashed underlines (available from nucleotide sequence with genbank accession number ak232603 ).; during the preparation of this manuscript a s scrofa chromosome 2 clone ch242-334a8 (working draft sequence; genbank accession number cu929919 ) without any assigned gene loci was released on september 24 2008.; the nucleotide sequences of the full-length cdna and the gene of porcine clec4g and the cdna of porcine cd23 reported in this paper have been deposited in the genbank database with accession numbers eu814900 eu814899 and fj545265 ."

"This project is funded in part by a grant from Fort Dodge Animal Health Inc. We thank Ms. Barbara Dryman for her assistance in plasmid extraction, and Dr. Stephen Wu for his encouragement and support."