Evaluating the conformation of recombinant domain I of β(2)-glycoprotein I and its interaction with human monoclonal antibodies.

"Disclosure The authors have no conflicting financial interests."

"We are indebted to Dr. Pojen Chen for donating the hybridoma line of native IS4. Monoclonal IgG expression vectors were a kind gift from Dr. Katy Kettleborough and Dr. Tarran Jones (Aeres Biomedical, London, UK). We are thankful to Dr. Siobhan ÒBrien and Dr. Alison Levy for their help and advice on the assembly of IgG constructs for expression. The murine monoclonal anti-DI antibody (mAb16) used in Western blot studies was a kind gift from Dr. Mike Iverson and Dr. Matt Linnik (La Jolla Pharmaceuticals, CA, USA). We are grateful for access to the MRC Centre for Biomedical NMR at Mill Hill. This work was supported by Arthritis Research, UK and the Oliver Bird Rheumatism Programme, Nuffield Foundation. The work in the Driscoll group is supported by the MRC (file reference U117574559). Dr. Ian Giles acknowledges support from the Rosetrees Foundation."