Analytical characterization and clinical evaluation of an enzyme-linked immunosorbent assay for measurement of afamin in human plasma.

"the within-run cv a value was retrieved from our precision study 2 9 reference values for the afamin assay were derived from 528 healthy blood donors at the red cross organization in linz austria as reported previously and as outlined in the supplementary data 2 10 in order to compare afamin concentrations between serum and plasma one serum sample and one edta-plasma sample were obtained from each of eight apparently healthy members of our laboratory staff (four males four females) after an overnight fast 2 11 in 25 patients (19 males and 6 females) with various diseases (eight of them with type 2 diabetes mellitus) a plasma sample was drawn at 07:00 a m following an overnight fast."

"Disclosures H. Dieplinger is owner and shareholder of Vitateq Biotechnology GmbH, a spin-off company of Innsbruck Medical University, holding several patents related to research described in this article. All other authors declare no conflicts of interest."

"This study was partly supported by a grant from the Austrian Science Fund (P19969-B11) to H.D. Vitateq Biotechnology GmbH provided reagents for afamin measurement free of charge and did not play a role in (1) the design of the study; (2) data collection, analysis and interpretation."