Dynamic changes in proteins during apple (Malus x domestica) fruit ripening and storage.

"table 1 identification of 53 differentially expressed protein spots in the apple proteome during ripening and senescence as determined by 2-de analysis maldi-tof-tof and mascot analysis spot no a protein name b gdr accession no c mascot score d matched % cov e matched peptides f theo mr(kda)/pi g obse mr(kda)/pi subcellular location h energy and metabolism energy (gluconeogenesis photosynthesis) gluconeogenesis36 nadp-dependent malic enzyme(nadp-me) mdp0000268037 49 1 1 83 34/5 81 80 00/6 00 cytoplasm 40 triosephosphate isomerase (tim) mdp0000694943 431 32 7 27 49/5 76 29 97/6 30 cytoplasm photosynthesis8 oxygen-evolving enhancer protein1 (oee1) mdp0000248920 444 38 8 35 29/6 09 34 19/5 51 chloroplast metabolism (carbohydrate amino acid nucleotide thiamine lipid and secondary) carbohydrate metabolism 10 probable 6-phosphogluconolactonase 2 (6pgl 2) mdp0000223309 109 7 5 34 08/5 12 31 73/5 43 chloroplast 19 soluble inorganic pyrophosphatase(ppase) mdp0000281233 407 11 6 64 36/5 35 29 79/5 62 cytoplasm 43 putative uncharacterized protein mdp0000127723 328 19 4 40 73/5 71 49 93/6 31 nuclear 35 beta-galactosidase mdp0000416548 425 14 10 81 52/5 69 79 87/6 07 apoplast 46 beta-galactosidase 3 mdp0000030527 66 1 1 95 63/8 34 79 73/6 15 apoplast amino acid metabolism 20 l-asparaginase 1 mdp0000658649 334 17 5 34 63/5 21 27 93/5 79 endoplasmic reticulum 27 cysteine synthase mdp0000325786 340 22 7 41 19/7 60 41 35/5 66 chloroplast stroma chromoplast 34 probable sarcosine oxidase mdp0000686885 577 23 7 44 10/5 59 44 26/6 09 cytoplasm 44 isovaleryl-coa dehydrogenase 2 (ivd 2) mdp0000225981 408 25 8 58 20/6 11 42 73/6 24 mitochondrion nucleotide metabolism 2 adenine phosphoribosyltransferase 1 (aprt 1) mdp0000121897 625 76 7 13 00/5 03 27 84/5 17 chloroplast cytoplasm 6 ump/cmp kinase (ump/cmpk) mdp0000240174 320 21 7 28 49/5 69 28 59/5 32 cytoplasm 7 adenine phosphoribosyltransferase 1 (aprt 1) mdp0000137185 616 42 8 27 00/8 40 28 00/5 48 chloroplast cytoplasm thiamine metabolism 24 thiazole biosynthetic enzyme (thi1) mdp0000206098 714 27 9 39 30/5 68 34 48/5 67 chloroplast lipid metabolism 25 epoxide hydrolase 2 (eh 2) mdp0000161121 349 16 8 35 34/5 20 40 38/5 61 cytoplasm peroxisome secondary metabolism 45 chavicol o-methyltransferase (cvomt 1) mdp0000219062 314 21 5 42 82/5 89 43 53/6 17 cytoplasm stress response and defense 1 ferritin-3 mdp0000189389 296 14 4 35 46/5 69 28 93/5 18 chloroplast 3 thioredoxin h-type (trx-h) mdp0000415439 435 43 7 13 88/5 39 16 30/5 51 cytoplasm 5 mlp-like protein 328 mdp0000132530 132 22 4 17 83/5 13 21 60/5 45 cytoplasm 16 superoxide dismutase [cu-zn] mdp0000258717 380 23 3 22 80/6 09 18 01/5 60 chloroplast 17 glycine-rich rna-binding protein (grp1a) mdp0000797759 412 52 8 16 88/5 61 17 17/5 68 nucleus21 26 5 kda heat shock protein mdp0000435717 364 16 6 37 21/9 34 28 17/5 76 mitochondrion 22 l-ascorbate peroxidase 2 mdp0000210077 625 20 8 37 95/5 71 31 71/5 73 cytoplasm 23 putative lactoylglutathione lyase mdp0000319112 401 29 8 39 97/6 40 35 32/5 60 cytoplasm 28 superoxide dismutase [cu-zn] mdp0000250286 517 29 4 22 28/6 28 18 30/5 83 chloroplast 33 abscisic acid stress ripening protein homolog mdp0000253074 211 11 1 25 72/6 01 30 64/6 05 nuclear 37 superoxide dismutase [cu-zn] 1 mdp0000201158 173 42 2 80 44/7 88 18 62/6 11 cytoplasm 39 17 8 kda class i heat shock protein mdp0000791550 261 31 5 17 52/5 99 21 42/6 27 cytoplasm 41 abscisic acid response protein mdp0000868045 414 31 4 19 86/8 61 33 55/6 29 nuclear 47 outer membrane lipoprotein blc (temperature-induced lipocalin) mdp0000148808 145 20 6 21 47/6 18 24 96/6 40 cell outer membrane 49 abscisic acid response protein mdp0000868045 257 17 3 19 86/8 62 32 46/6 43 nuclear 50 superoxide dismutase [mn] mdp0000187714 444 13 7 60 57/8 51 27 00/6 60 mitochondrion matrix 51 glutathione s-transferase mdp0000096349 632 57 9 24 03/6 17 28 13/6 58 cytoplasm 52 abscisic acid response protein mdp0000868045 397 31 4 19 86/8 68 31 09/6 65 nuclear 53 universal stress protein (usp) family protein mdp0000639609 130 10 1 19 81/6 30 23 34/6 68 cytoplasm 55 peroxiredoxin-2f mitochondrial (prxiif) mdp0000258515 161 15 2 21 61/8 77 23 90/6 85 mitochondrion matrix 4 major allergen pru ar 1 mdp0000288293 340 33 5 17 61/5 10 19 28/5 35 cytoplasm 15 major allergen mal d 1 mdp0000216907 444 38 5 17 52/5 54 18 47/5 53 cytoplasm 18 mlp-like protein 329 mdp0000277802 383 31 4 17 83/5 41 23 27/5 68 cytoplasm 31 major allergen mal d 1 mdp0000942516 566 28 6 17 53/5 62 20 27/5 82 cytoplasm 38 major allergen mal d 1 mdp0000942516 592 52 9 17 53/5 62 20 68/6 10 cytoplasm 54 thaumatin-like protein 1a mdp0000223025 85 1 2 12 26/5 71 22 79/6 68 extracellular ripening and senescence 12 1-aminocyclopropane-1-carboxylate oxidase 1 (acc oxidase 1) mdp0000195885 617 39 9 35 56/5 24 38 74/5 53 cytoplasm 13 1-aminocyclopropane-1-carboxylate oxidase 1 (acc oxidase 1) mdp0000195885 547 42 9 35 56/5 24 39 08/5 44 cytoplasm 14 1-aminocyclopropane-1-carboxylate oxidase 1 (acc oxidase 1) mdp0000195885 593 42 9 35 56/5 25 39 72/5 76 cytoplasm signal transduction 9 14-3-3 protein 7 mdp0000270640 207 13 4 30 54/5 31 32 77/5 42 cytoplasm 48 gtp-binding nuclear protein ran-3 mdp0000130864 66 8 2 28 54/6 14 31 30/6 53 cytoplasm cell structure 11 pollen-specific leucine-rich repeat extensin-like protein 3 (atpex3/pollen-specific lrr) mdp0000158152 45 2 1 60 12/6 37 31 19/5 47 cell wall 30 actin-depolymerizing factor 2 (adf 2) mdp0000245712 277 27 4 23 07/6 84 21 90/5 99 extracellular protein synthesis29 eukaryotic translation initiation factor 5a-2 mdp0000201872 308 42 6 17 76/5 46 21 48/5 81 cytoplasm 32 proteasome subunit beta type-1 mdp0000193666 55 4 1 34 19/8 07 28 22/6 02 cytoplasm nucleus a numbering corresponds to the 2-de gel in fig 1 b names of the proteins obtained via the mascot software v2 1 against the apple est database from genome database for rosaceae (gdr) ( http://www rosaceae org/species/malus/malus_x_domestica/genome_v1 0 ) c accession number from the apple est database from gdr d total ion score for the entire protein and for ions complemented by 100% of the confidence index (c i) e percent sequence coverage f numbers of matchedr peptides g theoretical molecular mass (mr) and isoelectric point (pi) of the homologous protein available at the apple est database observed molecular mass (mr) and isoelectric point (pi) estimated in comparison to the 2-de gel with marker proteins h subcellular location of each spot is based on the uniprot database."

"The authors declare no conflict of interest."

"This research was financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institution."