The evolution, diversity, and host associations of rhabdoviruses.

"raw read data were deposited in the ncbi sequence read archive (srp057824). data are available through figshare. diptera (flies mostly drosophilidae) were collected in the field from spain usa kenya france ghana and the uk ( supplementary data s1 ).; we extracted rna from seventy-nine individual insects (details in supplementary data s1 ) using trizol reagent (invitrogen) and combined the extracts into two pools (retaining non-pooled individual rna samples).; second for the virus sequences in the butterfly pararge aegeria and the medfly ceratitis capitata we were able to obtain infected samples to confirm whether sequences are only present in rna by performing pcr on both genomic dna and cdna as described above (samples kindly provided by casper breuker/melanie gibbs and philip leftwich respectively) all available rhabdovirus-like sequences were downloaded from genbank (accessions in supplementary data s2 )."

"Conflict of interest. None declared."

"Funding B.L. and F.M.J. are supported by a NERC grant (NE/L004232/1), a European Research Council grant (281668, DrosophilaInfection), a Junior Research Fellowship from Christ’s College, Cambridge (B.L.). G.G.R.M. is supported by an MRC studentship. The metagenomic sequencing of viruses from D.immigrans, D.tristis and S.deflexa was supported by a Wellcome Trust fellowship (WT085064) to D.J.O."