Single-cell analysis of the fate of c-kit-positive bone marrow cells.

"by the whole transcriptome sequencing 1551 differentially expressed genes (degs) which showed a statistically significant ( p < 0 05) fold-change difference >= 2 were found (fig 4 ; supplementary dataset s1 ); in myogenic c-kit-bmcs735 and 816 genes were upregulated and downregulated respectively.; the statistical significance ( p < 0 05) of the fold change (fc) difference is indicated d volcano plot representing gene expression levels in the two cell groups myogenic c-kit-bmcs were characterized by enrichment of the c-kit transcript (fig 5a ; supplementary dataset s1 ).; the expression pattern of cd63 and cd81 in myogenic c-kit-bmcs appeared to promote c-kit signaling as documented by the upregulation of the target gene mitf (fig 5a ; supplementary dataset s1 ).; moreover downregulation of the microtubule destabilizer stathmin (fig 5b ; supplementary dataset s1 ) may enhance the duplication and motility of c-kit-bmcs.; degs were subjected to gene ontology (go) for their functional classification; the top 10 terms of go functional analysis are shown for each category in fig 5c (see also supplementary dataset s1 ).; the enrichment heatmap involved six major biological processes comprising several subcategories (fig 5d ; supplementary figure s4 ) in which similarly and differentially expressed gene networks were identified by applying the modified fisher's exact text followed by bonferroni and fdr analysis (supplementary dataset s1 ).; supplemental material supplementary figure s1 supplementary figure s2 supplementary figure s3 supplementary figure s4 supplementary figure s5 supplementary figure s6 supplementary figure s7 supplementary figure s8 supplementary figure s9 supplementary figure s10 supplementary figure s11 supplementary figure s12 dataset 1 rna sequencing data anna czarna fumihiro sanada and alex matsuda contributed equally to this work."

"Competing interests P.A. is a member of Autologous Regeneration LLP. P.A. and A.L. are members of AAL Scientifics Inc. The remaining authors declare no competing financial interests."

"This study was supported by grants from the National Institutes of Health (NIH)."