Framework Mutations of the 10-1074 bnAb Increase Conformational Stability, Manufacturability, and Stability While Preserving Full Neutralization Activity.

"The authors would like to thank Dr. Michel Nuessenzweig for providing the antibody sequences and for his direction and insightful discussion and Dr. Jill Horowitz for her many useful discussions and program management. The authors thank Christian Mayer, in Dr. Nussenzweig’s lab, for providing the polyreactivity data, Dean Pettit at Just Biotherapeutics for critical reading of the manuscript, and Alex Taylor at Just Biotherapeutics for developing programs for in silico analysis. The authors are indebted to Pervin Anklesaria, of the Bill and Melinda Gates Foundation, for many hours of discussion and providing direction during the course of the studies. The authors also thank Tara McNair and Sam McMillan for expert technical assistance and David Beaumont for data management with the mouse pharmacokinetic studies. Finally, the authors gratefully acknowledge funding from the Bill and Melinda Gates Foundation to both Just Biotherapeutics (grant number OP1138851) and to the CAVIMC (grant number OP114996) at Beth Israel Deaconess Medical Center and Duke University."