Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling.

"the selective metabolic labelling of nucleic acids amino acids and key ev proteins such as the tetraspanins could enable further understanding of ev compositional changes that occur in response to different stimuli facilitating advances in ev production and clinical application as therapeutic drug delivery vehicles + electronic supplementary information (esi) available: https://github com/conor-horgan/ev-raman-analysis supplementary information supplementary movie acknowledgements c h and j p acknowledge funding from the nanomed marie sklodowska-curie itn from the h2020 programme under grant number 676137. the selective metabolic labelling of nucleic acids amino acids and key ev proteins such as the tetraspanins could enable further understanding of ev compositional changes that occur in response to different stimuli facilitating advances in ev production and clinical application as therapeutic drug delivery vehicles + electronic supplementary information (esi) available: https://github com/conor-horgan/ev-raman-analysis supplementary information supplementary movie acknowledgements c h and j p acknowledge funding from the nanomed marie sklodowska-curie itn from the h2020 programme under grant number 676137."

"Conflicts of interest: There are no conflicts to declare."

"C. H. and J. P. acknowledge funding from the NanoMed Marie Skłodowska-Curie ITN from the H2020 programme under grant number 676137. A. N. and M. M. S. acknowledge support from the GlaxoSmithKline Engineered Medicines Laboratory. T. E. W. acknowledges support from the EPSRC and Rosetrees Trust. V. N. acknowledges support from the Ermenegildo Zegna Founder’s Scholarship program and the Rosetrees Trust. L. M. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant number 642414. UK acknowledges support from the Deutsche Forschungsgemeinschaft [KA 4370/1-1]. M. S. B. acknowledges support from H2020 through the Individual Marie Skłodowska-Curie Fellowship “IMAGINE” (701713). M. M. S. acknowledges a Wellcome Trust Senior Investigator Award (098411/Z/12/Z) and the Engineering and Physical Sciences Research Council (EPSRC) programme grant “Advanced Functional Materials” (EP/M020398/1). We also acknowledge use of microscopy facilities within the Harvey Flower Electron Microscopy Suite, Department of Materials, Imperial College London. Raw research data is available upon request from rdmenquiries@imperial.ac.uk. "