Transcription and DNA Methylation Patterns of Blood-Derived CD8⁺ T Cells Are Associated With Age and Inflammatory Bowel Disease But Do Not Predict Prognosis.

"all microarray data have been deposited in arrayexpress accession numbers: e-mtab-7923 (expression data) and e-mtab-8925 (methylation data).; illumina gene expression arrays publicly available raw expression bead array data gse87650 15 were downloaded from geo datasets using geoquery version 2 50 5 e1 robust spline normalization of log-transformed raw data was performed using the lumi package version 2 34 0 18 and quality control was performed using arrayqualitymetrics version 3 34 0 19 samples failing quality control were removed and batch correction was performed using "combat" (part of sva version 3 26 0) 20 data were annotated using the lumihumanidmapping package.; transcript and dna methylation profiling all generated array data have been deposited in arrayexpress accession e-mtab-7923 (gene transcription arrays) and e-mtab-8925 (dna methylation data)."

"Conflicts of interest The authors disclose no conflicts."

"Funding This study was supported by grants from the Broad Medical Research Program at Crohn’s and Colitis Foundation of America, 10.13039/501100004815Isaac Newton Trust / Wellcome Trust ISSF and University of Cambridge Joint Research Grant Scheme, Cambridge Biomedical Research Campus pump priming grant and Action Medical Research UK. Marco Gasparetto was supported by fellowships from the Crohn’s in Childhood Research Association charity, the European Society for Paediatric Gastroenterology, Hepatology and Nutrition, and the IG-IBD International Exchange Program. Daniel R. Zerbino and Rachel D. Edgar were supported by core funding from the European Molecular Biology Laboratory. Peter Sarkies was supported by a grant from the UK Medical Research Council (Epigenetics and Evolution)."