HspB1 Overexpression Improves Life Span and Stress Resistance in an Invertebrate Model.

"hspb1 is well-conserved among chordates ( ) but there are potentially significant differences in invertebrate orthologs including the absence of the serine phosphorylation domain in caenorhabditis elegans hsp-25 ( supplementary data 1 ).; life tables of independent replicates and full statistics are shown in supplementary data 2 .; we identified 588 differentially expressed genes with an adjusted p -value < 05 ( figure 2 supplementary figure 4 and supplementary data 3 ).; additional replicates and statistics are included in supplementary data 2 .; surprisingly skn-1 rnai reduced hspb1::gfp abundance by a third while neither hsf-1 nor daf-16 rnai had any significant effect ( supplementary data 5 ).; all 3 collagen rnai's shortened the life span of hspb1 worms significantly more than control ( p < 01; figure 3d-f ; see supplementary data 2 for full statistics).; caenorhabiditis elegans does have a small heat shock protein orthologous to hspb1; however there are significant differences in the sequence including the absence of the serine phosphorylation site that is conserved among chordates ( supplementary data 1 ).; crystallin domain ( supplementary data 1 ).; most genes that have been reported upregulated dependent on daf-16 ( ) including the canonical targets mtl-1 and sod-3 ( ) were not upregulated in hspb1 worms compared with controls ( supplementary data 4 )."

"Conflict of Interest None declared."

"Funding This work was supported by funds from National Institutes of Health (U.S. Public Health Service Grant K12 GM111726) [CMC] and the National Institute on Aging (R00 AG049940) [KAR]. Some C elegans strains were provided by the Caenorhabditis Genetics Center, which is funded by National Institutes of Health Office of Research Infrastructure Programs (P40 OD010440)."