SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis.

"h f lw j cr-l m s and w j gm supervised the study and drafted the manuscript 13 tailored variant analysis pipeline can be found on https://github com/jordycoolen/easyseq_covid19 sars-cov-2 metadata and gisaid is available in the supplementary data 14 n/a 15 the easyseq(tm) rc-pcr sars-cov-2 wgs version 1 kit was supplied by nimagen and sequencing of the first two illumina libraries was performed by nimagen. detailed descriptions on the included samples can be found in supplementary data 4 sars-cov-2 rt-pcr was performed on all samples during routine diagnostics.; h f lw j cr-l m s and w j gm supervised the study and drafted the manuscript 13 tailored variant analysis pipeline can be found on https://github com/jordycoolen/easyseq_covid19 sars-cov-2 metadata and gisaid is available in the supplementary data 14 n/a 15 the easyseq(tm) rc-pcr sars-cov-2 wgs version 1 kit was supplied by nimagen and sequencing of the first two illumina libraries was performed by nimagen."

"h f lw j cr-l m s and w j gm supervised the study and drafted the manuscript 13 tailored variant analysis pipeline can be found on https://github com/jordycoolen/easyseq_covid19 sars-cov-2 metadata and gisaid is available in the supplementary data 14 n/a 15 the easyseq(tm) rc-pcr sars-cov-2 wgs version 1 kit was supplied by nimagen and sequencing of the first two illumina libraries was performed by nimagen. h f lw j cr-l m s and w j gm supervised the study and drafted the manuscript 13 tailored variant analysis pipeline can be found on https://github com/jordycoolen/easyseq_covid19 sars-cov-2 metadata and gisaid is available in the supplementary data 14 n/a 15 the easyseq(tm) rc-pcr sars-cov-2 wgs version 1 kit was supplied by nimagen and sequencing of the first two illumina libraries was performed by nimagen."

"Declaration of Competing Interest The authors have no conflict of interest to disclose."

"15 Funding/support The EasySeq™ RC-PCR SARS-CoV-2 WGS version 1 kit was supplied by NimaGen and sequencing of the first two Illumina libraries was performed by NimaGen. Further sequencing was performed by the Department of Medical Microbiology at the Radboud university medical center for the purpose of using the technology in routine diagnostics and support the national surveillance program. Therefore, no other funding was applied for."