Proteogenomic analysis of the autoreactive B cell repertoire in blood and tissues of patients with Sjögren's syndrome.

"single-cell fastq files were deposited at the ncbi as bioproject id prjna742201 and prjna7883525. for more details about the study subjects see online supplemental methods10 1136/annrheumdis-2021-221604 supp1 supplementary data 10 1136/annrheumdis-2021-221604 supp12 supplementary data table 1 patient characteristics at time of first study biopsy patient code sex age (years) duration since diagnosis (years) igm-rf titre (ie /ml cut-off: 10 ml) igg titre (g/l cut-off: 16 g/l) essdai b005 f 59 30 170 18 4 b007 m 63 7 4500 25 11 b008 m 61 1 48 19 6 b009 f 30 10 280 26 2 b010 f 74 21 15 15 2 b011 f 46 2 148 22 3 hc1 f 59 - - - - hc2 f 60 - - - - hc3 f 23 - - - - hc4 m 30 - - - - hc5 m 81 - - - - hc6 f 82 - - - - hc7 f 80 - - - - essdai eular sjogren's syndrome disease activity index.; this implies that stereotypic rf selection is driven stochastically by tissue-specific factors10 1136/annrheumdis-2021-221604 supp5 supplementary data 10 1136/annrheumdis-2021-221604 supp7 supplementary data 10 1136/annrheumdis-2021-221604 supp8 supplementary data earlier studies suggest that ana may be derived from polyreactive b cell precursors that share sequence motifs.; these data give insight into the molecular mechanisms of autoreactive inflammation and malt lymphoma and help to analyse the clinical response to rtx treatment in individual patients10 1136/annrheumdis-2021-221604 supp9 supplementary data 10 1136/annrheumdis-2021-221604 supp10 supplementary data 10 1136/annrheumdis-2021-221604 supp11 supplementary data we would like to thank sjoukje mulder for monitoring the study and amrah weijn for analytical support. data availability statement data are available in a public open access repository. single-cell fastq files were deposited at the ncbi as bioproject id prjna742201"

"Competing interests: None declared."

"Funding: This project was executed based on a Clinical Fellowship grant from the Netherlands Organisation for Health Research and Development (ZonMw; 90714540 and 435005039). Part of this work was carried out on the Dutch national e-infrastructure with the support of SURF Cooperative. JJW was supported by an Australian National Health and Medical Research Council Early Career Fellowship (1090759) and a Flinders University Research Collaboration grant."