Customized bioreactor enables the production of 3D diaphragmatic constructs influencing matrix remodeling and fibroblast overgrowth.

"data availability data described in the manuscript are available from the corresponding authors on reasonable request. microarray dataset was deposited in https://www ncbi nlm nih gov/geo/query/acc cgi? acc=gse180636"

"Competing interests The authors declare no competing interests."

"This work was supported by Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Grant no. 19/09 to M.P.; Fondazione CARIPARO-Ricerca Pediatrica 2016–2018, Grant no. 17/08 to P.G.P.; Fondazione CARIPARO-Ricerca Pediatrica 2018–2020, Grant no. 20/17 to M.P.; Progetto Uni-Impresa 2018–2020, “BIOXEN” grant to P.G.P. Istituto di Ricerca Pediatrica Grant 19/03 and Fondazione CARIPARO-Ricerca Pediatrica 2016–2018, Grant no. 17/09 to S.B. P.D.C. is supported by NIHR Great Ormond Street Hospital Biomedical Research Centre, the CDH-UK and the Great Ormond Street Hospital Charity, UK. A.C. is supported by Fondazione Umberto Veronesi, Grant 4420. We would like to thank Marco Braggion for his technical support in calcium transient analyses."