Identifying genetic determinants of inflammatory pain in mice using a large-scale gene-targeted screen.

"our review of published phenotypes reported in the mouse genome informatics database (queried march 11 2021) revealed that 7 of the 13 genes ( abhd13 alg6 bc048562 cgnl1 cnrip1 oxa1l and tecpr2 ) had no non-impc mouse alleles registered; therefore these impc alleles represent novel strains. full geneweaver results are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; all unprocessed data are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; a complete list of all abnormal open-field parameters and the associated mp terms for the 27 abnormal strains is available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; all data sets and analyses are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; wild type control data are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ) and reveal that the testing protocols established by all 5 contributing centers permitted detection of hypersensitive and hyposensitive genetically altered mouse strains.; this included 98 genes associated with at least 1 piece of functional genomics data (eg cerebellum gene expression correlates for hot plate measured in bxd recombinant inbred strains or qtl for morphine antinociception on chromosome 9) 67 genes associated with at least 2 pieces21 genes with >=4 pieces and 12 lacking any functional genomics evidence of a role in pain (full geneweaver results are available as a zenodo repository at https://doi org/10 5281/zenodo 5178015 ).; the results of statistical comparisons for all 110 knockout strains are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; note that the equivalent table for all 110 genes tested is available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; a complete list of all abnormal open-field parameters and the associated mp terms for the 27 abnormal strains is available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; all data sets scripts and output are available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 ).; the full table of results for all 110 genes tested is available as a zenodo repository ( https://doi org/10 5281/zenodo 5178015 )."

"Conflict of interest statement The authors have no conflicts of interest to declare."

"Research reported in this publication was supported as follows: For JAX, support came from the Office of the Director of the NIH under Award Number UM1OD023222, in the form of the Pain Administrative Supplement grant UM1 OD023222-07-S1. For TCP and UCD, support came from the Office of the Director of the NIH under Award Number UM1OD023221, in the form of the Pain Administrative Supplement grant UM1OD023221-07-S1. For BCM and HAR, support came from the Common Fund, through the OSC/Office of the NIH Director, NIAMS, NCI, NINDS, NIDCD, NIEHS, NIDA, and the ORWH under Award Number UM1 HG006348, in the form of the Pain Administrative Supplement grant UM1 HG006348-07-S2. For HAR, additional support came to the Mary Lyon Centre from the Medical Research Council grant code A410. For EBI, support came from the European Molecular Biology Laboratory core funding and the NHGRI of the NIH under Award Number UM1HG006370. For LD and MP, support came from the Canadian Excellence Research Chairs Program (CERC9). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."