One-step extraction and determination of 513 psychoactive substances, drugs, and their metabolites from hair by LC-MS/MS.

"Declarations Conflict of interestAll authors have given approval to the final version of the manuscript. The authors declare no competing interests. Conflict of interest All authors have given approval to the final version of the manuscript. The authors declare no competing interests."

"Funding The project was supported by the European Union the European Regional Development Fund under the Intelligent Development Operational Program 2014–2020, Priority axis 1: Support for R&D works by enterprises, Measure 1.1: R&D projects of enterprises, Sub-measure 1.1.1 Industrial research and development work within the framework of project No. POIR.01.01.01-00-0023/16 titled: Development of the innovative Next Generation Drug Clear Test (NGDC Test) for the detection of the so-called afterburners and in hair, blood and urine."