Effects of short-term unloading and active recovery on human motor unit properties, neuromuscular junction transmission and transcriptomic profile.

"gene expression analysis was performed separately on two sets of genes identified as relevant for nmjs based on 12 pathways extracted from the molecular signature database msigdb (v 7 4) (subramanian et al ) and skeletal muscle ion channels (jurkat-rott & lehmann-horn ) (see supporting information data s1 data s2 and data s3 ).; nmj rna-seq dataset is available (see supporting information data s1 ).; gsea showed changes in some nmj-related gene sets at ls10 and ar21 but not comparing ls0 and ar21 values (see supporting information data s3 ).; the ion channel rna-seq dataset is available (supporting information data s2 ).; data s1 .; data s2 .; data s3 . data availability statement rna-seq datasets are available at the gene expression omnibus repository: https://www ncbi nlm nih gov/geo/query/acc cgi? acc=gse211204"

"The present study was conducted in accordance with the standards set by the latest revision of the Declaration of Helsinki , and was approved by the Ethics Committee of the Department of Biomedical Sciences of the University of Padova (Italy) (reference number HEC‐DSB/01‐18). Participants were informed about all the experimental procedures through an interview and an information sheet. Volunteers signed a written consent form and were allowed to drop out of the study at any stage. Competing interests The authors have no conflict of interest to declare."

"Funding The present study was funded by the Italian Space Agency (ASI), MARS‐PRE, Project, n. DC‐VUM‐2017‐006. The authors are grateful to ASI for granting these funds to allow all the experiments to be performed."