Chronic Intake of a Meal Including Alaska Pollack Protein Increases Skeletal Muscle Mass and Strength in Healthy Older Women: A Double-Blind Randomized Controlled Trial.

"Notes: Supported by Nippon Suisan Kaisha, Ltd (to HM).: Author disclosures: KU and EY are employed by Nihon Suisan Kaisha, Ltd. The funder had the following involvement in the study: resources, conceptualization, methodology. All other authors report no conflicts of interest.: Supplemental Tables 1–3 are available from the “Supplementary data” link in the online posting of the article and from the same link in the online table of contents at https://academic.oup.com/jn/.: Funding Disclosure This study was funded by Nippon Suisan Kaisha, Ltd, 2019: Abbreviations used: Akt, protein kinase B; APP, Alaska pollack protein; BW, body weight; CON, whey protein control; ICC, intraclass correlation coefficient; MNA-SF, Mini Nutritional Assessment-Short Form; mTOR, mammalian target of rapamycin; QOL, quality of life; SMI, skeletal muscle mass index; TEI, total energy intake."

"Notes: Supported by Nippon Suisan Kaisha, Ltd (to HM).: Author disclosures: KU and EY are employed by Nihon Suisan Kaisha, Ltd. The funder had the following involvement in the study: resources, conceptualization, methodology. All other authors report no conflicts of interest.: Supplemental Tables 1–3 are available from the “Supplementary data” link in the online posting of the article and from the same link in the online table of contents at https://academic.oup.com/jn/.: Funding Disclosure This study was funded by Nippon Suisan Kaisha, Ltd, 2019: Abbreviations used: Akt, protein kinase B; APP, Alaska pollack protein; BW, body weight; CON, whey protein control; ICC, intraclass correlation coefficient; MNA-SF, Mini Nutritional Assessment-Short Form; mTOR, mammalian target of rapamycin; QOL, quality of life; SMI, skeletal muscle mass index; TEI, total energy intake."

"This study was approved by the ethics committee of Tokushima University (approval number: 3417), and the trial was registered at the University Hospital Medical Information Network (UMIN000035718). Informed consent was obtained from all study participants, and written informed consent was obtained from them to publish this article. Data from a previous investigation were utilized for sample size estimation. The calculation was based on an effect size of 0.692 [absolute difference of 0.18 kg/m2 skeletal muscle mass index (SMI) with an SD of 0.26 kg/m2] (), α level of 0.05, and a power (1 − β) of 80%; we identified as necessary 34 individuals for each study group. We aimed to enroll ≥43 individuals/group to be conservative and to allow for potential dropouts and a missing data rate of 20%. Conclusions: Daily intake of a meal containing APP compared with whey protein increases skeletal muscle mass and lower-extremity muscle strength in healthy older women, suggesting that an APP-containing meal may be useful in the prevention of sarcopenia in this group.: This trial was registered at as UMIN000035718."