Single-Nuclei RNA Sequencing of 5 Regions of the Human Prenatal Brain Implicates Developing Neuron Populations in Genetic Risk for Schizophrenia.

"single-nuclei rna-seq fastq files are available through the european genome-phenome archive ( https://ega-archive org ) under study accession egas00001006537. the magma celltyping package ( https://github com/neurogenomics/magma_celltyping ) ( ) was used to test for enrichment of schizophrenia common variant genetic associations ( ) (available through: https://doi org/10 6084/m9 figshare 14672178 ) within each fetal brain cell population.; for comparison magma was also performed on gwas data for autism ( ) (available through: https://figshare com/articles/dataset/asd2019/14671989 ) and human height ( ) (available through: https://portals broadinstitute org/collaboration/giant/index php/giant_consortium_data_files ).; the gene expression matrix for each assayed brain region is provided through a publicly accessible figshare repository: https://figshare com/articles/dataset/11629311 ."

"the magma celltyping package ( https://github com/neurogenomics/magma_celltyping ) ( ) was used to test for enrichment of schizophrenia common variant genetic associations ( ) (available through: https://doi org/10 6084/m9 figshare 14672178 ) within each fetal brain cell population."

"Acknowledgments and Disclosures This work was supported by a project grant (Grant No. MR/T002379/1) and Centre grant (Grant No. MR/L010305/1) from the 10.13039/501100000265Medical Research Council (UK). We thank Dr. Joanne Morgan for assistance with sequencing. The human fetal material was provided by the Joint 10.13039/501100000265MRC/10.13039/100010269Wellcome Trust (Grant No. 099175/Z/12/Z) Human Developmental Biology Resource (http://www.hdbr.org). NJB and MCO have received a research award from 10.13039/100008373Takeda Pharmaceutical Company Ltd., unrelated to this work. All other authors report no biomedical financial interests or potential conflicts of interest. Data Access Statement: Gene expression specificity values for each cell population are provided in Supplemental tables. The gene expression matrix for each assayed brain region is provided through a publicly accessible figshare repository: https://figshare.com/articles/dataset/11629311. Single-nuclei RNA-Seq FASTQ files are available through the European Genome-Phenome Archive (https://ega-archive.org) under study accession EGAS00001006537."

"This work was supported by a project grant (Grant No. MR/T002379/1) and Centre grant (Grant No. MR/L010305/1) from the 10.13039/501100000265Medical Research Council (UK)."