SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene.

"the results are reported in a user-friendly table as well as in fastq format available as a command line interface from https://github com/kblin/covid-spike-classification or in a browser-based app running locally from https://ssi biolib com/app/covid-spike-classification/run without data leaving the computer."

"the results are reported in a user-friendly table as well as in fastq format available as a command line interface from https://github com/kblin/covid-spike-classification or in a browser-based app running locally from https://ssi biolib com/app/covid-spike-classification/run without data leaving the computer.; the program is available from https://github com/kblin/covid-spike-classification ."

"Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper."

"This work was supported by the Poul Due Jensen Fonden (Grundfos Fonden, Corona-Danica). T.S.J. and K.B. received support by The Novo Nordisk Foundation through the grants NNF-IIMENA (NNF16OC0021746) and NNF CFB (NNF20CC0035580). We are grateful to researchers, clinicians, and public health authorities for making SARS-CoV-2 sequence data available through GISAID. Tue Sparholt Jørgensen: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing. Martin Schou Pedersen: Data curation, Formal analysis, Investigation, Resources, Validation, Writing – review & editing. Kai Blin: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – review & editing. Franziska Kuntke: Data curation, Investigation, Validation, Visualization, Writing – review & editing. Henrik K. Salling: Conceptualization, Investigation, Methodology, Resources, Writing – review & editing. Rasmus L. Marvig: Data curation, Formal analysis, Investigation, Resources, Validation, Writing – review & editing. Thomas Y Michaelsen: Investigation, Resources, Writing – review & editing. Mads Albertsen: Resources, Writing – review & editing. Helene Larsen: Conceptualization, Funding acquisition, Investigation, Project administration, Supervision, Writing – review & editing."