Non-responsiveness to cardioprotection by ischaemic preconditioning in Ossabaw minipigs with genetic predisposition to, but without the phenotype of the metabolic syndrome.

"the raw data for these 8 ossabaw minipigs are available from the national center for biotechnology information (ncbi) sequence read archive/bioproject number prjna837405 (accession numbers: samn28202074-samn28202081).; thus we used the only publicly available representative reference genome assembly from ncbi for sus scrofa submitted by "the swine genome sequencing consortium" [ ] (sscrofa11 1; genbank: gca_000003025 6; refseq: gcf_000003025 6)."

"Declarations Conflict of interestP.K., H.L., A.S., A.G., S.R., and G.H. declare that they have no conflict of interest. M.S. and M.A. are both founders and Chief Scientific Officer and Chief Executive Officer, respectively, of CorVus Biomedical, LLC, which produces Ossabaw minipigs. Ethical approvalThe experimental protocols were approved by the Bioethical Committee of the district of Düsseldorf (G1610/17; G1777/20) and conform to the guidelines from Directive 2010/63/EU of the European Parliament on the protection of animals used for scientific purposes. We followed the ARRIVE guidelines 2.0 [60, 61]. Conflict of interest P.K., H.L., A.S., A.G., S.R., and G.H. declare that they have no conflict of interest. M.S. and M.A. are both founders and Chief Scientific Officer and Chief Executive Officer, respectively, of CorVus Biomedical, LLC, which produces Ossabaw minipigs."

"Funding Open Access funding enabled and organized by Projekt DEAL. This work was supported by the German Research Foundation [SFB 1116 B08 to G.H. and P.K.]; the European COST ACTION in CARDIOPROTECTION [CA16225 to G.H.]; and the U.S. National Institutes of Health [DK097512, M.A, M.S.]."