Regulatory T cells suppress CD4+ effector T cell activation by controlling protein synthesis.

"rna-seq data are deposited under the accession number gse171789 . utrs with higher tes identified by speed ( data s1 a-e ).; briefly 131 genes increased in te in stimulated cells relative to unstimulated cells at 5% false discovery rate (fdr) and also decreased in te in treg-exposed stimulated cells relative to the unstimulated cells ( data s1 a ).; untranslated mrna sequences for 127 sequences ( data s1 b ) using biomart ( http://uswest ensembl org/biomart/martview/e728a9d413ab0dfd039a3ce666b3d3e2 ).; we used the meme software tool to identify a de novo motif enriched in these sequences (considering both the positive and negative strand) and the top hit closely resembles a top motif ( data s1 c ) 109 of the 127 sequences have an instance of the motif with a p value of 0 01 or lower 82 of the 109 show the motif in the strand orientation we expect while 27 show the motif in the opposite orientation.; the motif was enriched at a p value of 0 000126 ( data s1 d ) and this tool identified 67 instances of the motif among the 127 sequences ( data s1 e ).; data s1 shows analysis and enrichment of top sequences.; data s1 shows analysis and enrichment of top sequences (a) te_stimvnostim_131_de csv; (b) meme_top_motif_gene_list txt; (c) meme_127_motif_output txt; (d) sea tsv; and (e) sea_topmotifsequences tsv."

"Disclosures: V.S. Muir reported “other” from Janssen R&D outside the submitted work. J.H. Buckner reported “other” from Gentibio and grants from Helmsley Charitable Trust outside the submitted work; in addition, J.H. Buckner had a patent to PCT/US2020/039445 pending and a patent to US 8,053,235 issued and is a Scientific Co-Founder and Scientific Advisory Board member of GentiBio, a company engaged in developing engineered regulatory T cell therapies. No other disclosures were reported."

"This project was funded by National Institutes of Health grants R21AI143227 to S.F. Ziegler and R. Savan and 5T32AI106677-07 (T32) and 1F32AI145283-01A1 (F32) training grant to L. So."