Mono(2-ethylhexyl) phthalate induces transcriptomic changes in placental cells based on concentration, fetal sex, and trophoblast cell type.

"availability of data and material rna sequencing data is available in the ncbi gene expression omnibus (geo) database under accession number gse217210. xlsx 860 kb) online resource 3 table availability of data and material rna sequencing data is available in the ncbi gene expression omnibus (geo) database under accession number gse217210"

"the code associated with this project is publicly available on github ( https://github com/slapehn/placentalcell_mehp_rnaseq )."

"Declarations Conflicts of interestThe authors declare that they have nothing to disclose. Ethics approvalPlacentas used for primary syncytiotrophoblasts were collected into a tissue repository under a protocol approved by the Oregon Health & Science University Institutional Review Board. Consent to participateAll patients that provided placentas gave informed consent for their participation. Consent for publicationAll authors have given their consent for submission of this content. Conflicts of interest The authors declare that they have nothing to disclose."

"The authors would like to acknowledge their funding sources, including the National Institute of Child Health and Human Development (K99/R00-HD096112) as well as the Brotman Baty Institute (Catalytic Collaboration Grant) (AGP). This study was also supported by resources from the University of Washington Interdisciplinary Center for Exposures, Diseases, Genomics, and Environment funded by the National Institute of Environmental Health Sciences (2P30ES007033). We would also like to thank the women who donated placental samples to the OHSU Placental Biorepository, Dr. David Beier for sharing laboratory space, and Anika Rajput for technical assistance. ECHO PATHWAYS is funded by National Institutes of Health (1UG3OD023271-01, 4UH3OD023271-03). The Conditions Affecting Neurocognitive Development and Learning in Early Childhood (CANDLE) study was funded by the Urban Child Institute and NIH (R01 HL109977). Author contributions: Funding and conception of the study was performed by AGP. The methodology was contributed to by SL, SH, KA, LK, LM, JWM, TKB, and AGP. Laboratory work related to the investigation was carried out by SL and SH with data analysis conducted by SL and AGP. Data visualization was designed and performed by SL and AGP. Resources and supervision for the project were provided by LM, SS, and AGP. The original draft of the manuscript was written by SL and AGP. SL, SH, KA, LK, JWM, TKB, KZL, LM, SS, and AGP participated in the review and editing of the manuscript and approved of its submission for publication."