A novel TNFRSF1A mutation associated with TNF-receptor-associated periodic syndrome and its metabolic signature.

"Disclosure statement : The authors have declared no conflict of interest."

"Funding This work was supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 834259). J.D.S., A.Annibal, R.L. and A.Antebi were supported by the Max-Planck-Gesellschaft (MPG). Disclosure statement: The authors have declared no conflict of interest. Ethics: The study was conducted in accordance with the Declaration of Helsinki, and samples were collected and analyzed under protocols approved by the ethics committee (vote 12–240), University of Cologne, Cologne, Germany. The study is registered at the German Clinical Trials Registry under the ID: DRKS00010534. Informed consent was received."

"Heparinized whole blood was obtained from study participants after written informed consent was given according to approval by the ethics committee (vote 12–240), University of Cologne, Cologne, Germany. The study is registered at the German Clinical Trials Registry under the ID: DRKS00010534. Plasma protein concentration was measured using a bicinchoninic acid (BCA) kit (Thermo Fisher Scientific, Bremen, Germany). Metabolites were extracted using the Folch Method as described previously []. Briefly, 200 µl of chloroform and 150 µl of methanol were added to the plasma. Samples were shaken for 1 h at 4°C, followed by centrifugation at 3000 r.c.f. for 10 min at 4°C. Funding: This work was supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 834259). J.D.S., A.Annibal, R.L. and A.Antebi were supported by the Max-Planck-Gesellschaft (MPG).: Disclosure statement: The authors have declared no conflict of interest.: Ethics: The study was conducted in accordance with the Declaration of Helsinki, and samples were collected and analyzed under protocols approved by the ethics committee (vote 12–240), University of Cologne, Cologne, Germany. The study is registered at the German Clinical Trials Registry under the ID: DRKS00010534. Informed consent was received."