Development and evaluation of a simple PCR assay and nested PCR for rapid detection of clarithromycin-resistant Helicobacter pylori from culture and directly from the biopsy samples in India.

"amplicon (bp) references2143f gta aac ggc ggc cgt aac tat 183 this study 2143ar gta aag gtc cac ggg gtc gt this study 2143gr gta aag gtc cac ggg gtc gc this study urebf cgt ccg gca ata gct gcc ata gt 480 [ ] urebr gta ggt cct gct act gaa gcc tta [ ] 23s rrna f ggc tct ttg agt cct ttt agg aca a 617 [ ] 23s rrna r ctc cat aag agc caa agc cct tac t [ ] in this work the sequence discovered were deposited in genbank under accession number (genbank accession number mw080351 to mw080363).; availability of data and materials the sequences submitted in the genbank are available with accession number mw080351 to mw080363. availability of data and materials the sequences submitted in the genbank are available with accession number mw080351"

"Declarations Ethics approval consent to participateThe ICMR-NICED Ethical committee had approved the study. Competing interestsThe authors affirm that there were no financial or commercial ties that might be viewed as a potential conflict of interest throughout the execution of the research. Competing interests The authors affirm that there were no financial or commercial ties that might be viewed as a potential conflict of interest throughout the execution of the research."

"Funding This study was supported in part by Department of Science and Technology (DST) and Indian Council of Medical Research (ICMR), Government of India. BCK acknowledges Indian Council of Medical Research (ICMR) for providing a Senior Research Fellowship (No. 5/3/8/36/ITR-F/2020)/Department of Science and Technology for providing fellowship (DST/INSPIRE Fellowship/2014/IF140909). SP acknowledges CSIR for furnishing fellowship (09/482(0065)/2017-EMR-I). Funders have no role in study design, data collection, analysis, interpretation and publication."