Secreted mammalian DNases protect against systemic bacterial infection by digesting biofilms.

"Disclosures: V.J. Torres reported grants from Jassen Biotech, Inc. and personal fees from Jassen Biotech, Inc. outside the submitted work. B. Reizis is an advisor for Related Sciences and a co-founder of Danger Bio, companies developing therapeutics for autoimmune diseases. No other disclosures were reported."

"We thank Suzane Rooijakkers (UMC Utrecht, Utrecht, Netherlands) for kindly providing the anti-WTA mAb, Sergei Koralov (New York University, New York, NY, USA) for the NET-containing control sample, and Alice Liang, Joseph Sall, Chris Petzold, and Kristen Dancel-Manning of the NYU Langone Health Division of Advanced Research Technologies Microscopy Lab for assistance with EM. Microscopy shared resource is supported by NIH grant CA016087 at the Laura and Isaac Perlmutter Cancer Center. The Gemini300 SEM with 3View was purchased with the support of NIH grant OD019974. The nuc mutant of S. aureus was obtained from the Nebraska transposon mutant library (Network of Antimicrobial Resistance in S. aureus program), which was supported under the NIH-National Institute of Allergy and Infectious Diseases contract HHSN272200700055C. This study was supported by the NIH grants AR071703 and AR070591 (B. Reizis), AI100853 (L. Serpas, A. Rashidfarrokhi); AR069515 and GM136573 (L. Serpas); AI137336, AI099394, AI105129; and AI121244 (V.J. Torres). It was also supported by the Lupus Research Alliance and the Colton Center for Autoimmunity (B. Reizis), the Uehara Memorial Foundation (S. Makita), and the Cystic Fibrosis Foundation (K.A. Lacey). V.J. Torres is a Burroughs Wellcome Fund Investigator in the pathogenesis of infectious diseases. Disclosures: V.J. Torres reported grants from Jassen Biotech, Inc. and personal fees from Jassen Biotech, Inc. outside the submitted work. B. Reizis is an advisor for Related Sciences and a co-founder of Danger Bio, companies developing therapeutics for autoimmune diseases. No other disclosures were reported."