Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection.

"data availability statement the authors confirm that the study data is available within the article and supplemental materials (openly available in figshare at https://doi org/10 6084/m9 figshare 22257727 v1 ). c)) (supplemental data 1) suggesting that the ability to thermoregulate is a strong predictor of survival ( figure 3 ).; c) suggesting both methods are relatively comparable (supplemental data 1).; c) as a humane endpoint in this study we would have accurately predicted mortality in 99 out of 100 (99 0%) animals that were ultimately fdic and survival of 68 out of 78 (87 2%) animals (supplemental data 1).; c) threshold the majority of false negative predictors of mortality came from the e cloacae (6 out of 14) s aureus (4 out of 14) and k pneumoniae (4 out of 14) groups (supplemental data 1).; c) as a humane endpoint in this study we would have accurately predicted mortality in 77 out of 100 (77 0%) animals that were ultimately fdic and survival of 69 out of 78 (88 5%) animals (supplemental data 1).; c) threshold the majority of false negative predictors of mortality came from the s aureus (11 out of 26) and e cloacae (7 out of 26) groups (supplemental data 1).; additional future analysis of clinical scoring sub-components (supplemental data 1) may be warranted to identify whether certain sub-components represent an outsized influence on mortality predictions. data availability statement the authors confirm that the study data is available within the article and supplemental materials (openly available in figshare"

"Disclosure statement No potential conflict of interest was reported by the author(s)."

"RD, YA, and DZ were involved in protocol development and study design; ME, WWS, and TF prepared bacterial pathogens; RD, YA, RA, and CC were involved in performing animal observations; RD and TW were responsible for data analysis and figure generation; RD, TW, ME, and DZ were responsible for the writing of the manuscript; all authors were involved in editing and review of the manuscript. We thank Sridhar Samineni and Yoann LeBreton for assistance with animal use protocol development; Tesfaye Mekennon, and Kifle Workagegnehu for performing animal observations; Joe Anderson and Dan Finnegan for histopathological tissue analysis; and all members of the WRAIR/NMRC animal care and husbandry teams, especially Alphonso Evans, for their support of our animals throughout the entire study. This work was supported by the Military Infectious Diseases Research Program under grant W0383_20_WR."