Acetylation of histone H2B marks active enhancers and predicts CBP/p300 target genes.

"data availability this project's sequencing raw data processed peak regions and gene tracks are available on the ncbi geo under accession no gse186349 . reporting summary peer review file supplementary data 1 the sequence of the targeting vector used to generate the hdac1/2-gfp-fkbp12 f36v cells. data availability this project's sequencing raw data processed peak regions and gene tracks are available on the ncbi geo under accession no gse186349"

"Competing interests The authors declare no competing interests."

"We thank the members of the Choudhary laboratory for their helpful discussions. We thank E. Maskey for her excellent technical assistance. The Novo Nordisk Foundation Center for Protein Research is financially supported by the Novo Nordisk Foundation (no. NNF14CC0001). C.C. was supported by the Novo Nordisk Foundation (nos. NNF14OC0008541 and NNF22OC0074677). This work was funded by the European Commission FP7 grant (no. SyBoSS FP7-242129). Y.H. was supported by a Grant‐in‐Aid for JSPS Overseas Postdoctoral Fellows. S.K. was supported by a Lundbeck Foundation Fellowship (no. R347-2020-2170). We thank the SyBoSS partners K. Anastassiadis, F. Steward, A. Smith and W. Skarnes for sharing E14TG2a Oct4-IRES-Puro mouse ESCs. We thank Abcam, Cell Signaling Technology, Thermo Fisher Scientific, Active Motif and RevMAb Biosciences for providing some of the antibody samples for testing. We thank the CPR Imaging Platform, the CPR Big Data Management Platform and the CPR and DanStem Genomics Platform for their assistance. We thank the ENCODE Consortium and the ENCODE production laboratory for generating and sharing the data. We also thank Fulco et al.44 and Gasperini et al.47 for providing unrestricted access to their data."