Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphins.

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"This work was supported by a grant from China Academy of Chinese Medical Sciences Innovation Fund (ID: CI2021A03404) and National Natural Science Foundation of China (ID: 82130122, ID: 81973964 and ID: 81674083). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. Data availability: All data included in this study are available on request by contact with the corresponding author. Author contributions: X.-Y. Wang and X.-H. Jing conceived and designed the study. J.-t. Shi performed the experiment of flow cytometry, Western blot, and the quantitative real-time PCR. W.-y. Cao performed the animal experiments, acquired the blood samples, tissue dissection and processing, immunohistochemistry, and confocal imaging. H.-Y. Wan analyzed the data of flow cytometry. X.-N. Zhang analyzed the data of RNA sequencing. Z.-Y. Qu offered insights and assisted in interpreting the results. Y.-S. Su and W. He wrote the article. R. Wang supervised the project and revised the article. All the authors provided feedback and approved the article. Special thanks are extended to Gong Zhou and Zhi-Yuan Hui, who engaged in the experiment of behavioral experiment. This work was supported by a grant from China Academy of Chinese Medical Sciences Innovation Fund (ID: CI2021A03404) and National Natural Science Foundation of China (ID: 82130122, ID: 81973964 and ID: 81674083). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. Data availability: All data included in this study are available on request by contact with the corresponding author. Author contributions: X.-Y. Wang and X.-H. Jing conceived and designed the study. J.-t. Shi performed the experiment of flow cytometry, Western blot, and the quantitative real-time PCR. W.-y. Cao performed the animal experiments, acquired the blood samples, tissue dissection and processing, immunohistochemistry, and confocal imaging. H.-Y. Wan analyzed the data of flow cytometry. X.-N. Zhang analyzed the data of RNA sequencing. Z.-Y. Qu offered insights and assisted in interpreting the results. Y.-S. Su and W. He wrote the article. R. Wang supervised the project and revised the article. All the authors provided feedback and approved the article. Special thanks are extended to Gong Zhou and Zhi-Yuan Hui, who engaged in the experiment of behavioral experiment."