Functional specialization of short-lived and long-lived macrophage subsets in human tonsils.

"data from macaque tonsils are available at geo ( gse207261 ).; data from human tonsil macrophages are available at geo ( gse207260 ).; data availability sequencing data are available from geo: scrna-seq data from macaque mononuclear phagocytes from tonsils ( gse207261 ) and rna-seq data from human tonsil macrophages ( gse207260 ). data availability sequencing data are available from geo: scrna-seq data from macaque mononuclear phagocytes from tonsils ( gse207261"

"Disclosures: The authors declare no competing interests exist."

"This work was funded by Institut National de la Santé et de la Recherche Médicale, Institut Curie (CIC IGR-Curie 1428), the Agence Nationale de la Recherche (ANR-10-LABX-0043, ANR-10-IDEX-0001-02 PSL), Institut National du Cancer (2018-1-PL BIO-01-1), and Fondation ARC pour la Recherche sur le Cancer (PJA 20171206249) and TRANSVAC2 H2020 (grant #730964). Department of IDMIT (Fontenay-aux-Roses, France) infrastructure is supported by fundings of the “Programme Investissements d’Avenir” managed by the ANR under reference ANR-11-INBS-0008. J. Villar is a fellow of the IC-3i PhD program supported by the ERC Horizon 2020-Marie Sklodowska-Curie Actions (grant #666003) and a fellow of the Fondation pour la Recherche Médicale (FDT202106013025)."

"Adult cynomolgus macaques (Macaca fascicularis), aged 36 mo and originating from Mauritian Association for Assessment and Accreditation of Laboratory Animal Care–certified breeding centers, were used in this study. All macaques were housed in the Department of Infectious Disease Models and Innovative Therapies (IDMIT) infrastructure facilities (Commissariat à l'énergie atomique et aux énergies alternatives, Fontenay-aux-Roses), under BSL-2 and BSL-3 containment when necessary (animal facility authorization D92-032-02, Prefecture des Hauts de Seine, France) and in compliance with European Directive 2010/63/EU, the French regulations and the Standards for Human Care and Use of Laboratory Animals of the Office for Laboratory Animal Welfare (assurance number A5826-01). The protocols were approved by the institutional ethical committee “Comité d’Ethique en Expérimentation Animale du Commissariat à l’Energie Atomique et aux Energies Alternatives” (CEtEA 44) under statement number A19_031. The study was authorized by the Research, Innovation and Education Ministry under registration number APAFIS#21359-2019070510474423 v1."