Validation of a Transcriptome-Based Assay for Classifying Cancers of Unknown Primary Origin.

"Declarations FundingFunding for this project and open access was provided directly by Tempus Labs. Conflicts of interestAll authors are employees, former employees, and/or hold restricted stock units of Tempus Labs, Inc. Availability of data and materialRaw data for this study were generated at Tempus Labs. Derived data supporting the findings of this study are available within the paper and its Supplementary Figs./Tables. Requests for additional data should be addressed to publication.inquiry@tempus.com. Due to the clinical nature of these samples, requests will be evaluated by a Data Access Review Group and are subject to a signed data transfer agreement. Ethics approvalAnalyses were performed using de-identified data under the exemption Pro00042950, granted from the Advarra, Inc Institutional Review Board (IRB) on 15 April 2020 based on the Department of Health and Human Services regulations found at “45 CFR 46.104(d)(4).” All methods were carried out in accordance with relevant guidelines and regulations. Internal validations and laboratory protocols were developed and performed in a CLIA-certified, CAP-accredited laboratory. Consent to participateWritten informed consent is not required from the subject/s or legal guardians per the above-stated exemption (consent was exempted by Advarra Inc. Institutional Review Board). Consent for publicationN/A. Code availabilityN/A. Author contributionsJM, AB, JK, AJH, JG, KAB, and TT conceptualized the work. BM performed wet-lab experiments. JM, KM, NB, MS, RJ, EM, KAB, and TT curated data. MB, AB, JK, KM, CM, and KAB wrote the software. JM and JK provided the formal analysis and validation. MCS, NB, MS, RJ, EM, KB, JG, KAB, and TT supervised the work. JM, KM, AJH, JG, KAB, and TT contributed to data visualization. All authors participated in drafting the manuscript. JM, JK, KM, AJH, CI, JTD, MCS, NB, JG, KAB, and TT edited the manuscript. Conflicts of interest All authors are employees, former employees, and/or hold restricted stock units of Tempus Labs, Inc."

"Funding Funding for this project and open access was provided directly by Tempus Labs."