Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry.

"the ms proteomics data have been deposited to the proteomexchange consortium via the pride partner repository with the dataset identifier pxd029305 [ ] 2 6 full scan ms spectra ( m/z 375-1500) were acquired in positive mode at a resolution of 120000 at m/z 200 a maximum injection time of 60 ms and an automatic gain control (agc) target value of 3 x 10 6 ."

"Declaration of competing interest The authors declare that there are no conflicts of interest."

"This project was supported by the “10.13039/501100003032Association Nationale de la Recherche et de la Technologie” and UCB Pharma S.A. (Belgium and France) via the CIFRE fellowship of Steve Hessmann. This work was supported by the “Agence Nationale de la Recherche” via the French Proteomic Infrastructure ProFI FR2048 (ANR-10-INBS-08-03). The authors thank Laura Herment and Tanguy Fortin from Anaquant for their support in the use of the HCP Profiler standard."