A pilot metabolomic study of drug interaction with the immune response to seasonal influenza vaccination.

"data availability the metabolomics data have been submitted to metabolomics workbench and publicly available under study id st002432"

"Competing interests The authors declare no competing interests."

"This research was conducted while JMB was Diamond/American Federation for Aging Research (AFAR) award recipient. This work was in parted funded by NIH grants NCI U01 CA235493 (SL), NIAID R01 AI149746 (SL), NIA P30AG067988 (SL and JMB, the UConn Claude D. Pepper Older Americans Independence Center). ANC was supported by a NIAMS/NIH predoctoral fellowship (T32AR079114). We thank Drs Haoxin Li, Marios Giannakis and Kevin Bullock for providing metabolite identification information in the Broad dataset."

"All participants were vaccinated with Fluzone high-dose trivalent flu vaccine (Sanofi Pasteur Inc., Swiftwater, PA) after ~70 days of treatment. Blood was drawn via standard venipuncture into EDTA-treated vacutainers prior to treatment (Day 0), prior to vaccination (~day 35 and ~day 70), and 7, ~35, and ~70 days post vaccination. The study protocol was approved by the Institutional Review Board at the University of Connecticut Health Center (UCHC) and registered at ClinicalTrials.gov (NCT03996538). All study participants provided written informed consent to participate in the study."