Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges.

"table 5 list of main bioinformatics tools with their features and types of cas proteins tool name organism cas nuclease enzyme characteristics website references crispresso2 human and mouse cas9 cpf1 amplicon sequencing and its interpretation for genome editing http://crispresso2 pinellolab org/submission [ ] cas-analyzer (crispr-ga) human mouse rat drosophila zebrafish nmcas9 spcas9 sacas9 stcas9 ascpf1/lbcpf1 editing genomes and creating synthetic nucleases (programmable nucleases) https://crispr-ga net/ [ ] crispor human arthropoda cas9 orthologues cas variants primer sets genomic location of targets and numerous predictive models http://crispor tefor net/ [ ] tide/tider - spcas9 sacas9 st1cas9 nmcas9 ascpf1 fncpf1 lbcpf1 large-scale induced mutations in the editing site are identified with a custom deconstruction approach http://shinyapps datacurators nl/tider/ [ ] chopchop human mouse zebrafish bacteria cas9 cas12 cas13 talen providing multiple predictive models; visualizing genomic location of targets and genes; and providing primers https://chopchop cbu uib no/ [ ] crispr rgen tools human cas9 microhomology-predictor predicts out-of-frame scores predicts probable off-target numbers and can be used independently http://www rgenome net/ [ ] e-crisp human spcas9 genome-scale library construction in a practical manner; freely available and regularly updated http://www e-crisp org/e-crisp/ [ ] crisprscan human cas9 cas12 coding-gene sgrna design and genome browser tracks https://www crisprscan org/ [ ] cctop human mouse model cas9 easy to use highly customizable single and multiple query searching; identifying mismatches; estimating off-target impacts; sgrna efficiency prediction https://cctop cos uni-heidelberg de:8043/ [ ] crista - spcas9 offering a machine learning framework for identifying false positives and prioritizing targets which is more precise than existing methods https://crista tau ac il/ [ ] deepcrispr human spcas9 predicting off-target effects by factoring in epigenetic data https://github com/bm2-lab/deepcrispr [ ] wu-crispr human mouse spcas9 offering an ml algorithm that has been highest prediction scores https://bio tools/wu-crispr [ ] crisprz zebrafish human mouse spcas9 specific for a wide range of species and cell lines https://research nhgri nih gov/crisprz/?"

"table 5 list of main bioinformatics tools with their features and types of cas proteins tool name organism cas nuclease enzyme characteristics website references crispresso2 human and mouse cas9 cpf1 amplicon sequencing and its interpretation for genome editing http://crispresso2 pinellolab org/submission [ ] cas-analyzer (crispr-ga) human mouse rat drosophila zebrafish nmcas9 spcas9 sacas9 stcas9 ascpf1/lbcpf1 editing genomes and creating synthetic nucleases (programmable nucleases) https://crispr-ga net/ [ ] crispor human arthropoda cas9 orthologues cas variants primer sets genomic location of targets and numerous predictive models http://crispor tefor net/ [ ] tide/tider - spcas9 sacas9 st1cas9 nmcas9 ascpf1 fncpf1 lbcpf1 large-scale induced mutations in the editing site are identified with a custom deconstruction approach http://shinyapps datacurators nl/tider/ [ ] chopchop human mouse zebrafish bacteria cas9 cas12 cas13 talen providing multiple predictive models; visualizing genomic location of targets and genes; and providing primers https://chopchop cbu uib no/ [ ] crispr rgen tools human cas9 microhomology-predictor predicts out-of-frame scores predicts probable off-target numbers and can be used independently http://www rgenome net/ [ ] e-crisp human spcas9 genome-scale library construction in a practical manner; freely available and regularly updated http://www e-crisp org/e-crisp/ [ ] crisprscan human cas9 cas12 coding-gene sgrna design and genome browser tracks https://www crisprscan org/ [ ] cctop human mouse model cas9 easy to use highly customizable single and multiple query searching; identifying mismatches; estimating off-target impacts; sgrna efficiency prediction https://cctop cos uni-heidelberg de:8043/ [ ] crista - spcas9 offering a machine learning framework for identifying false positives and prioritizing targets which is more precise than existing methods https://crista tau ac il/ [ ] deepcrispr human spcas9 predicting off-target effects by factoring in epigenetic data https://github com/bm2-lab/deepcrispr [ ] wu-crispr human mouse spcas9 offering an ml algorithm that has been highest prediction scores https://bio tools/wu-crispr [ ] crisprz zebrafish human mouse spcas9 specific for a wide range of species and cell lines https://research nhgri nih gov/crisprz/?"

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"Funding Not applicable."