Transgene-free direct conversion of murine fibroblasts into functional muscle stem cells.

"these authors contributed equally: x q i k data availability the bulk and single-cell rna sequencing data generated as part of this study are available in gene expression omnibus (geo) with the accession number gse208064. data availability the bulk and single-cell rna sequencing data generated as part of this study are available in gene expression omnibus (geo) with the accession number gse208064"

"Competing interests O.B.-N. is an inventor on a pending patent related to the production of iMPCs filed by the General Hospital Corporation (Pub. No. US20230042917A1, Appl. No. US17/846,556, filed June 22, 2022). The remaining authors declare no competing interests."

"The authors are grateful to Shahragim Tajbakhsh for providing the Pax7-nGFP mouse strain and Nadezhda Rotankova for assisting with small molecule compound screens. We acknowledge the use of the Functional Genomics Center Zurich (FGCZ) facility and infrastructure and are grateful to their staff members for their assistance with next generation library preparation and Illumina sequencing. We thank Lukas Graz from the Seminar for Statistics at ETH Zurich for assistance with statistical analyses. We thank Ajda Lenardič and Veerle de Goederen for providing feedback to the manuscript. We further acknowledge the use, under a purchased license, of the software BioRender in several schematics. This work was supported by an Eccellenza Grant from the Swiss National Science Foundation (grant number PCEGP3_187009) as well as a grant from The Novartis Foundation for Medical-Biological Research. Other supports to the Bar-Nur lab include grants from the Good Food Institute, Helmut Horten and Olga Mayenfisch foundations. All authors read and approved the final manuscript version."