Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype.

"Declarations Ethics approval and consent to participateAll the experimental procedures involving animals were following the National Research Council’s Guide for the Care and Use of Laboratory Animals [61] and were approved by the Cairo University Research Ethics Committee (REC-FOPU) and the October University for Modern Sciences and Arts (MSA) research ethics committee under reference number (M1/EC1/2020PH). Consent for publicationNot applicable. Competing interestsThe authors declare that they have no competing interest. Competing interests The authors declare that they have no competing interest."

"Funding Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB). This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors."