Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung.

"code availability code for analysis of scrna-sequencing data is available at: https://github com/jason-spence-lab/frum-et-al ."

"code availability code for analysis of scrna-sequencing data is available at: https://github com/jason-spence-lab/frum-et-al ."

"Competing interests The authors declare no competing interests but the following competing financial interests: J.R.S. holds intellectual property rights pertaining to lung organoid technologies, and T.F. and J.R.S. have filed a provisional patent application for lung organoid technologies, describing methods of alveolar type 2 differentiation from primary cells and iPSCs based on the findings in the current study. The remaining authors declare no competing financial or non-financial interests."

"This study was made possible by funding from the following sources: CZF2019-002440 from the Chan Zuckerberg Initiative DAF, an advised fund from the Silicon Valley Community Foundation and by NIH-NHLBI R01HL119215 and NHLBI R01HL166139 to J.R.S. T.F. was additionally supported by NIH NIDCR T32DE007057. P.P.H is additionally supported by the Rogel Cancer Center Fellowship. R.F.C.H. is additionally supported by NIH-NHLBI F31HL152531. A.C.S. was supported by 5T32GM007863 and is currently supported by NIH-NHLBI F30HL156474. I.A.G is supported by NIH-NICHD 5R24HD000836. J.Z.S. is supported by NIH-NIDDK R01DK120623. We thank the University of Michigan Advanced Genomics core, the University of Michigan Microscopy core and the University of Michigan Flow Cytometry core for assistance with experiments in this manuscript. We also acknowledge the University of Washington Laboratory of Developmental Biology for access to lung tissue. Additionally, we acknowledge current members of the Spence Laboratory for helpful insight and discussion during this study and Lindy K. Brastrom for critical reading of the manuscript."