Wide field-of-view fluorescence imaging for organ-level lineage tracing of rare intestinal stem cell populations.

"code data and materials availability data and code used for analysis is publicly available in the following figshare repository: https://doi org/10 25422/azu data 24166266 ethics statement the animal study was reviewed and approved by iacuc protocol 18-440 phs animal welfare assurance number: d16-00159 (a-3248-01) university of arizona."

"Disclosures The authors have no relevant financial interests in the manuscript and no other potential conflicts of interest to disclose."

"ND performed all imaging studies and data analysis. SD performed animal necropsies for the second imaging study and performed tissue fixation, sectioning and fluorescence microscopy. HS performed mouse necropsies for the first imaging study. NL assisted with imaging system development and operation. RS oversaw all animal care and assisted with all mouse necropsies. JLM and TWS provided guidance on the experimental design and interpretation of results. ND, SD, and TWS contributed to the first draft of the manuscript. All authors reviewed and approved of the final manuscript. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health (Grant No. P30 CA023074), National Science Foundation (Grant No. DGE-2137419), Department of Defense (Grant No. W81XWH-22-1-0211) and the University of Arizona Cancer Center Cancer Engineering Fellowship."