Blocking Tim-3 enhances the anti-tumor immunity of STING agonist ADU-S100 by unleashing CD4⁺ T cells through regulating type 2 conventional dendritic cells.

"Competing Interests: The authors have declared that no competing interest exists."

"We thank Dr. Jinming Gao from the Nano-Immuno-Oncology (U54) Center of UT Southwestern Medical Center for detailed comments and discussion on the manuscript; Dr. Hailong Wang from the Tianjin Medical University Cancer Institute and Hospital for the timely help in data analysis of the mIHC staining; Dr. Bowen Ding and Mr. Zhaosong Wang from the Tianjin Medical University Cancer Institute and Hospital for assistance with the experiments of in vivo adoptive transfer of immune cells. This study is funded by National Natural Science Foundation of China (82303196, 82373279, 82373283, 82302913, U20A20375, 82103001), Haihe Laboratory of Cell Ecosystem Innovation Fund (22HHXBSS00004), China Postdoctoral Science Foundation (2022M712387), Tianjin Natural Science Foundation (21JCQNJC01430) and Tianjin Key Medical Discipline (Specialty) Construction Project (TJYXZDXK-009A)."