Low Mutation Load in a Supergene Underpinning Alternative Male Mating Strategies in Ruff (Calidris pugnax).

"for analysis of potential donor species for the introgression analysis we additionally included all resequencing data from other calidris species available from ncbi bioproject #prjna419629.; finally we included 1 outgroup species a interpres available from ncbi bioproject #prjna545868 (sra#: srr9946666 and srr9946658) that is part of the b10k project ( ).; data availability the sequence data generated in this study and genome assemblies have been submitted to ncbi ( http://www ncbi nlm nih gov/bioproject/prjna816664 ). data availability the sequence data generated in this study and genome assemblies have been submitted to ncbi ( http://www ncbi nlm nih gov/bioproject/prjna816664"

"genome annotation is available online ( https://github com/leifanderssonlab/ruff_assembly_2022/annotation_gff ).; code associated with bioinformatic analyses are available online ( https://github com/leifanderssonlab/ruff_assembly_2022 )."

"Conflict of interest statement. None declared."

"We thank Russel Corbett-Detig, Peter Grant, and Rosemary Grant for comments on the manuscript, Mats Pettersson for technical advice, and Mateusz Konczal and Fyodor Kondrashov for access to Calidris data. The project was financially supported by Vetenskapsrådet (2017-02907 to L.A.), Knut and Alice Wallenberg Foundation (KAW 2016.0361 to L.A.), the National Science Foundation of China (81961128002 to Y.L.), and the Basal Research Grant of South China Institute of Environmental Sciences (No. 22060302001001124; to C.W.). The National Genomics Infrastructure (NGI)/Uppsala Genome Center provided service in massive parallel sequencing, and the computational infrastructure was provided by the Swedish National Infrastructure for Computing (SNIC) at UPPMAX partially funded by the Swedish Research Council (2018-05973)."