Single-cell RNA sequencing of a new transgenic t(8;21) preleukemia mouse model reveals regulatory networks promoting leukemic transformation.

"data availability the scrna-seq data has been uploaded to the gene expression omnibus (geo) under accession gse173712. data availability the scrna-seq data has been uploaded to the gene expression omnibus (geo) under accession gse173712"

"Competing interests The authors declare no competing interests."

"The authors first thank Ella Kothari and Jun Zhao at the University of California, San Diego Mouse Transgenic Core Shared Resource for their help in generating the Rosa26 AE9a knock-in mouse model. Additionally, the authors thank the University of California, San Diego Moores Cancer Center Flow Cytometry Core Facility staff for assistance in analyzing bone marrow HSPC populations and the Institute for Genomic Medicine (IGM) core facility staff for performing the 10x Genomics library preparation and the associated scRNA-seq. The authors also thank Dr. Roman Sasik at the University of California, Center for Computational Biology and Bioinformatics (CCBB) for performing the initial Cell Ranger processing of the scRNA-seq data. Finally, the authors thank Dr. Nathan Salomonis and Dr. H. Leighton Grimes at Cincinnati Children’s Hospital for guidance in using the cellHarmony data processing package. This work was supported by National Institutes of Health (NIH) grants R01CA104509 (DEZ) and T32CA009523 (ML). The University of California, San Diego Moores Cancer Center Core facilities are supported by the National Cancer Center Support Grant (CCSG P30CA23100)."