Quorum sensing modulates bacterial virulence and colonization dynamics of the gastrointestinal pathogen <i>Citrobacter rodentium</i>.

"data availability statement rna-seq data generated from this study are available on the ncbi sequence read archive (sra) under the bioproject accession number prjna935367. we identified that in the absence of ahl production there were 92 transcripts differentially upregulated and 125 transcripts differentially downregulated ( figure 2a ; differential expression data are available in supplementary data 3).; gene set enrichment analysis (gsea) identified an enrichment in pathways related to bacterial secretion systems and to flagellar assembly as well as a downregulation of genes associated with starch and sucrose metabolism ( figure 2b data available in supplementary data 5).; croi strain were strongly associated specifically with the type iii secretion (t3s) system and associated effectors ( figure 2b supplementary data 6).; differential expression data are available in supplementary data 3 (b) pathway enrichment analyses were calculated by using gene set enrichment analysis (gsea; shown in blue); and overrepresented kegg modules (shown in orange) were calculated by using clusterprofiler.; supplementary enrichment data are available in supplementary data 5-6 (c) heatmap showing z-score expression of genes associated with the type iii secretion system.; general alignment and quality statistics are available in supplementary data 1.; gene counts were imported into r (v4 1 0) for further processing (supplementary data 2).; 1 25 (supplementary data 3).; pathway enrichment analysis was performed by using gene set enrichment analysis (gsea v4 2 3) using a pre-ranked list of all genes within the dataset (after pre-filtering) arranged according to the wald-test statistic (supplementary data 4).; enrichment analysis data are available in supplementary data 5-6. data availability statement rna-seq data generated from this study are available on the ncbi sequence read archive (sra) under the bioproject accession number prjna935367"

"Disclosure statement No potential conflict of interest was reported by the author(s)."

"The authors would like to thank all of our colleagues in the Finlay laboratory for their support and assistance. B.B.F. is a University of British Columbia Peter Wall Distinguished Professor. J Peña-Díaz received support from the University of British Columbia (UBC) and from Mitacs. Supporting images were created with BioRender (BioRender.com). This research was enabled in part by software provided by the Digital Research Alliance of Canada (alliancecan.ca). We are grateful to Matthew Croxen and Kirsten Koymans for generating the DBS100 ∆croI mutant. We would also like to thank Lisa Thorson for the fundamental logistical support of the project. Author contributions: J.P.D. and B.B.F conceived the project. J.P.D., S.E.W., A.S.P., A.C.C., W.D., and S.O.J. performed and designed experiments. J.P.D., S.E.W., and A.C.C., analyzed data. J.P.D. wrote bioinformatics pipelines. J.P.D. wrote the original draft of the manuscript with input from all authors. All authors revised the manuscript. B.B.F. acquired funding for the project and provided supervision."