Role of affinity in plasma cell development in the germinal center light zone.

"the computer code to process the antibody sequences is available on github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ) scrna-seq (smartseq2) data from this study ( fig 4 and fig 5 ) are available at the gene expression omnibus under accession no gse246382 . the raw sequencing data and computer scripts associated with fig 1 and fig 5 have been deposited at github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ).; the computer code to process the antibody sequences is available on github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ) scrna-seq (smartseq2) data from this study ( fig 4 and fig 5 ) are available at the gene expression omnibus under accession no gse246382 ."

"heavy and light chains derived from the same cell were subsequently paired and clonotypes were assigned using the publicly available pipeline at github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ).; the raw sequencing data and computer scripts associated with fig 1 and fig 5 have been deposited at github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ).; the computer code to process the antibody sequences is available on github ( https://github com/stratust/igpipeline/tree/igpipeline2_timepoint_v2 ) scrna-seq (smartseq2) data from this study ( fig 4 and fig 5 ) are available at the gene expression omnibus under accession no gse246382 ."

"Disclosures: The authors declare no competing interests exist."

"This work was supported by the National Institutes of Health (NIH) (1P01AI138212, P01AI100148, and 5R37 AI037526), NIH Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery (1UM1AI144462-01), MC Bulgari 2023, and the Stavros Niarchos Foundation to M.C. Nussenzweig. M.C. Nussenzweig is a Howard Hughes Medical Institute investigator."