The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes.

"data availability all primary sequencing data have been deposited as paired-end fastq files and all mapped data have been deposited as bigwig files in the gene expression omnibus under the accession number gse224292 . libraries were amplified by addition of 2 ul each of barcoded 10 mm i5 and i7 primer solutions (supplementary data 1 ) and nebnext hifi 2 x pcr master mix (neb cat no m0541) with 13 rounds of amplification as described previously .; libraries were amplified by addition of 2 ul each of barcoded 10 mm i5 and i7 primer solutions (supplementary data 1 ) and nebnext hifi 2x pcr master mix (neb cat no m0541) with gap-filling and 12-cycle pcr: 58 ?; libraries were prepared for illumina sequencing with udi (unique dual indexes) adapters (supplementary data 1 ) without size-selection and following the kapa dna polymerase library preparation kit protocol ( https://www kapabiosystems com/product-applications/products/next-generation-sequencing-2/dna-library-preparation/kapa-hyper-prep-kits/ ) optimized to favor exponential amplification of <1000-bp fragments over linear amplification of large dna fragments as described previously : 98 ?; reporting summary peer review file supplementary data 1 high-throughput sequencing primer information and indexes."

"Competing interests The authors declare no competing interests."

"We thank K. Ahmad and T. Tsukiyama for critical readings of the manuscript, J. Henikoff for help with processing of sequencing data, S. Showman for help with western blotting experiments, and D. Scalzo and X. Wang (Northwestern University) for guidance on mESC culturing. This research was supported by NIH grant no. K99 GM138920 (S.B.), the Howard Hughes Medical Institute (S.H.) and NIH grant no. P30CA015704 (Fred Hutch Shared Resources)."