Metaproteomic assessment of gut microbial and host functional perturbations in <i>Helicobacter pylori</i>-infected patients subjected to an antimicrobial protocol.

"data availability statement the mass spectrometry proteomics data (including peptide identification quantification and annotation tables) have been deposited to the proteomexchange consortium via the pride 79 partner repository with the dataset identifier pxd042946. summed abundance values measured for taxa and taxon-specific functions in the 10 patients at t0 t1 and t2 were then compared using a paired t-test (see methods for further details about the data analysis workflow; results of data aggregation and differential analyses are provided in supplementary dataset s1).; in the second analysis only the host proteins were considered and their abundance values were normalized to obtain identical total abundances at all time points (abundance and differential data are given in supplementary dataset s2).; other members of proteobacteria showed an opposite variation with a decreased relative abundance in t1 (i e pseudomonadales; supplementary dataset s1).; of these 222 219 and 210 functions respectively were significantly reduced at t1 while 52 45 and 34 functions respectively were increased (supplementary dataset s1).; twelve proteobacteria functions were decreased at t1 (dataset s1) including enzymes involved in amino acid metabolism (ketol-acid reductoisomerase glutamate carboxypeptidase alanine dehydrogenase and glutamate dehydrogenase) and sulfur-based energy metabolism (taurine-pyruvate aminotransferase sulfate adenylyltransferase 3'-phosphoadenosine 5'-phosphosulfate synthase dissimilatory sulfite reductase also detected as significantly reduced when considering desulfovibrionales-specific peptides).; at t0 when each patient was still colonized by h pylori 22 functions were relatively more abundant than at t1 (supplementary dataset s2)."

"Disclosure statement No potential conflict of interest was reported by the author(s)."