Functional Prokaryotic-Like Deoxycytidine Triphosphate Deaminases and Thymidylate Synthase in Eukaryotic Social Amoebae: Vertical, Endosymbiotic, or Horizontal Gene Transfer?

"Conflict of Interest The authors have no competing interests to declare."

"This work was supported by the University of Nebraska Foundation that included the Susan O. Prather Memorial Fund, Blair Paxton Udale Fund for Life Sciences, Mary D. Rogick Memorial Fund, and School of Biological Sciences 3M Lehr Fund as Special Funds for Graduate Students awarded to H.L. We thank Dr. Paul Russell and Dr. Oliver Limbo (Scripps Research, La Jolla) for providing the dCMP deaminase knockout and WT S. pombe strains, Dr. Mark Wilson and Dr. Peter Madzelan (University of Nebraska, Lincoln) for the pREP3X vector, and Dr. Curtiss and Dr. Soo-Young (University of Florida) for providing the WT E. coli strain χ2842. We thank Terri Fangman and Microscopy Core Research Facility (University of Nebraska, Lincoln) for helping with confocal imaging of the E. coli cells. We thank Dr. Jeremy Wideman (personal communication; Arizona State University) for the method to efficiently use EukProt sequences. We also thank Drs. Greg Somerville, Limei Zhang, Wei Niu, and Wayne Riekhof for advice about growth experiments."