Multiomics Analyses With Stool-Type Stratification in Patient Cohorts and Blautia Identification as a Potential Bacterial Modulator in Type 2 Diabetes Mellitus.

"all data were deposited to the national center for biotechnology information sequence read archive under bioproject number prjna795858."

"Duality of Interest. No potential conflicts of interest relevant to this article were reported."

"This work was supported by the National Key Research and Development Program of China (2021YFC2300300), the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (ZYYCXTD-D-202001), and the National Natural Science Foundation of China (81430097, 81973837, 32070667, 31671366, and 32300078)."

"We recruited 103 patients with new-onset, untreated T2DM and 25 HCs who met specific inclusion criteria (Supplementary Material). Patients, who were initially screened from 1,798 individuals in Beijing from July 2015 to December 2016, self-evaluated their stools with the initial guidance of clinicians and went through a 2-week screening performed by clinicians. To ensure that we recorded the representative and stable stool type for each patient over time, we finally enrolled patients with T2DM who self-reported consistently dry stool (DM-DS) or loose stool (DM-LS) over the past 3 months and maintained unchanged stool type at the beginning and end of the screening (DM-LS, n = 79; DM-DS, n = 24). HCs with normal stools were healthy volunteers who attended routine examinations in Guang’anmen Hospital from February to May 2016. The clinicians assessed the consistency of the collected stool samples using Bristol Stool Scale scores and confirmed the enrollment of all participants. The study followed the principles of the Declaration of Helsinki, was approved by the ethics committee of Guang’anmen Hospital (No. 2015EC060-02), and is registered in the Chinese Clinical Trial Registry (ChiCTR-IOR-15006626). The blood, stool, and urine samples of each participant were collected for the subsequent multiomics detection ( and Supplementary Material)."