Folate deficiency reduced aberrant level of DOT1L-mediated histone H3K79 methylation causes disruptive SHH gene expression involved in neural tube defects.

"availability of data and materials datasets used in this study have been deposited in gene expression omnibus (geo) database and can be accessed through https://www ncbi nlm nih gov/geo/info/linking html with geo series record gse238081."

"Declarations Ethics approval and consent to participateBoth animal and human samples used in this study are approved by the Ethics Board of Capital Institute of Pediatrics with Ethics Review Number DWLL2021011 and SHERLLM2021024. The human samples were secondary utilization of the previous samples with exception to the requirement of informed consent. We have attached the Ethical approval of the previous study. Competing interestsThe authors declare no competing interests. Competing interests The authors declare no competing interests."

"We are delighted in assistance and clinical data from participating hospitals. We are grateful for Dr Jeremy Allen from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), to amend our manuscript. This work was supported by the National Natural Science Foundation Projects (82071690, 81971390), Research Foundation of Capital Institute of Pediatrics (FX-2020-05, CXYJ-2-21-09), Public service development and reform pilot project of Beijing Medical Research Institute (BMR2021-3)."