The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis.

"the previously established oarsi histopathological scoring system [ ] from 0 to 6 was applied (supplementary data s1 ) by two blinded investigators (ah and tm).; primer sequences of assessed genes can be found in supplementary data s2 .; a voi was placed around the subchondral mtp using the same technique as for the femoral head (supplementary data s3 ).; the tibial epiphysis was evaluated using a separate voi excluding the subchondral bone plate and avoiding the medial and lateral cortical bone and growth plates (supplementary data s3 )."

"Declarations Ethics approval and consent to participateThis study was approved by the internal animal welfare committees and the number of animals duly notified to the competent authority (Landesamt für Gesundheit und Soziales, Berlin, Germany) (T-CH0009/20). Consent for publicationNot applicable. Competing interestsThe authors declare that they have no competing interests. Competing interests The authors declare that they have no competing interests."

"Funding Open Access funding enabled and organized by Projekt DEAL. This work was supported by the Stiftung Oskar-Helene-Heim (OHH) to TM; the Else Kröner-Fresenius-Stiftung (EKFS 2017_A22) to JK; the Deutsche Forschungsgemeinschaft (DFG KE 2179/2–1, TS 303/2–1) to JK and ST. This study was partly funded by the DFG Collaborative Research Center 1444 and the European Union under Grant Agreement Nr. 101095635 (PROTO). Views and opinions expressed are however those of the authors only and do not necessarily reflect those of the European Union or the European Health and Digital Executive Agency (HADEA). Neither the European Union nor the granting authority can be held responsible for them."