Multistep tumor genetic evolution and changes in immunogenicity trigger immune-mediated disease eradication in stage IV melanoma: lessons from a single case.

"data availability statement the gene expression dataset of tumor samples is deposited in gene expression omnibus with accession number gse171484. these results indicate that tumor genetic evolution was shaped by treatment with the brafi which led to the selection of melanoma subpopulations carrying mutations related to acquired brafi resistance 10 1136/jitc-2023-007612 supp2 supplementary data 10 1136/jitc-2023-007612 supp1 supplementary data figure 2 genetic and transcriptional profiles of t1-t4 tumor samples (a) pathogenic gene variants detected by targeted mutation analysis performed by the ion torrent personal genome platform (thermofisher scientific) (b) copy number alteration (cna) genomic plots obtained by analysis by oncoscan cnv arrays (affymetrix). data availability statement the gene expression dataset of tumor samples is deposited in gene expression omnibus with accession number gse171484"

"Competing interests: None declared."

"Funding: Ministero della Salute: RCR2020 to ACC Melanoma Working Group, and Ricerca Corrente; AIRC IG17462; CARIPLO Foundation (2015-0911, VV support)."